plantsЗащита растенийPlant Protection0135-3705Институт защиты растенийplants-191Research ArticleФИТОПАТОЛОГИЯPHYTOPATHOLOGYБиохимические параметры гуанилатциклазной системы как индикатор устойчивости растений томата к фитофторозуBiochemical parameters of guanylate cyclase system as biomarker of tomato resistance to the late blightБакакинаЮ. С.BakakinaY. S.

г.Минск

Minsk

СодельД. Л.SodelD. L.

г.Минск

Minsk

ДубовскаяЛ. В.DubovskayaL. V.

г.Минск

Minsk

ВолотовскийИ. Д.VolotovskiI. D.

г.Минск

Minsk

Институт биофизики и клеточной инженерии НАН БеларусиInstitute of Biophysics and Cell Engineering NAS of Belarus201612062022040159167Copyright © Бакакина Ю.С., Содель Д.Л., Дубовская Л.В., Волотовский И.Д., 20222022Бакакина Ю.С., Содель Д.Л., Дубовская Л.В., Волотовский И.Д.Bakakina Y.S., Sodel D.L., Dubovskaya L.V., Volotovski I.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://plants.belal.by/jour/article/view/191

В статье представлены результаты исследований по сравнительному анализу биохимических параметров гуанилатциклазной системы – содержание циклического гуанозинмонофосфата (цГМФ) и фермента его синтеза гуанилатциклазы (ГЦ), в контрастных по устойчивости к патогену Phytophthora infestans сортах томата OttaWa 30 и Доходный. Установлена обратная зависимость между устойчивостью к патогену и уровнем содержания цГМФ в растениях. Впервые детектированы белки, подобные ГЦ животных, в субклеточных фракциях растений томата, выявлены сортовые отличия. Полученные данные могут выступать дополнительными критериями при отборе сортов растений томата, устойчивых к патогенам.

The results on comparative analysis of guanylate cyclase system biochemical parameters, i.e. the content of cyclic guanosine 3’,5’-monophosphate (cGMP) and the content of guanylate cyclase (GC) enzyme, in tomato cultivars OttaWa 30 and Dohodny with contrast resistance to the pathogen Phytophthora infestans, are presented. It was demonstrated that endogenous cGMP content in biotic stress-tolerant plants was lower than in stress-sensitive ones. Proteins similar to GC of mammalian were detected in subcellular fractions of tomato plants for the first time. The content of GC-like proteins was differed in stress-tolerant and stress-sensitive plants. The findings could be used as additional criterions for the selection of tomato plants resistant to pathogens.

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The authors declare that there are no conflicts of interest present.