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Biochemical parameters of guanylate cyclase system as biomarker of tomato resistance to the late blight

Abstract

The results on comparative analysis of guanylate cyclase system biochemical parameters, i.e. the content of cyclic guanosine 3’,5’-monophosphate (cGMP) and the content of guanylate cyclase (GC) enzyme, in tomato cultivars OttaWa 30 and Dohodny with contrast resistance to the pathogen Phytophthora infestans, are presented. It was demonstrated that endogenous cGMP content in biotic stress-tolerant plants was lower than in stress-sensitive ones. Proteins similar to GC of mammalian were detected in subcellular fractions of tomato plants for the first time. The content of GC-like proteins was differed in stress-tolerant and stress-sensitive plants. The findings could be used as additional criterions for the selection of tomato plants resistant to pathogens.

About the Authors

Y. S. Bakakina
Institute of Biophysics and Cell Engineering NAS of Belarus
Belarus

Minsk



D. L. Sodel
Institute of Biophysics and Cell Engineering NAS of Belarus
Belarus

Minsk



L. V. Dubovskaya
Institute of Biophysics and Cell Engineering NAS of Belarus
Belarus

Minsk



I. D. Volotovski
Institute of Biophysics and Cell Engineering NAS of Belarus
Belarus

Minsk



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For citations:


Bakakina Y.S., Sodel D.L., Dubovskaya L.V., Volotovski I.D. Biochemical parameters of guanylate cyclase system as biomarker of tomato resistance to the late blight. Plant Protection. 2016;(40):159-167. (In Russ.)

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ISSN 0135-3705 (Print)